|uveal melanoma (UM)
|normal uveal tissues; UM cell lines OCM-1, M619 and MuM-2B
|qRT-PCR, MTT assay, cell cycle assay, transwell assay, luciferase reporter assay, Western blot
|Overexpression of microRNA-21 promoted proliferation, migration, and invasion of OCM-1, M619 and MuM-2B cells. Wild type p53 was identified as a target gene of microRNA-21-3p, and proved by dual luciferase reporter assay. Up-regulated microRNA-21 inhibited the expression of wild type p53 gene, and the increased expression of LASP1 in mRNA level and protein level.
|Role of microRNA-21 in uveal melanoma cell invasion and metastasis by regulating p53 and its downstream protein.
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