|uveal melanoma (UM)
|human RPE cell line ARPE-19, human normal liver cell line L‐02, human UM cell lines: SP6.5 and OM431
|Northern blot, qPCR, luciferase reporter assay, immunoblot, ELISA, Hoechst 33342 staining, histological analysis, TUNEL assay
|To improve uveal melanoma specificity of adenovirus-based TRAIL introduction, we used miRNA response elements (MREs) of miR-34a, miR-137 and miR-182 to regulate its expression. We constructed an MRE-directed TRAIL-expressing adenoviral vector and provided evidence that this vector possessed high anti-tumor activity and uveal melanoma specificity.
|Tumor-targeting TRAIL expression mediated by miRNA response elements suppressed growth of uveal melanoma cells.
Copyright © Institution of Biomedical Big Data, Wenzhou Medical University All rights reserved.