|lens epithelial cells; human lens epithelial cell line SRA01/04
|DNA sequencing, qRT-PCR, luciferase reporter assay, flow cytometry, Western blot
|TP53 was identified as a target of miR-125b, and the minor allele of the rs78378222 polymorphism promoted the miR-125b/TP53 mRNA interaction. Transfection with 50 nM miR-125b mimics markedly reduced the mRNA and protein expression levels of TP53 in the cultured lens epithelial cells, and miR-125b significantly induced apoptosis in the epithelial cells compared with negative control cells.
|rs78378222 polymorphism in the 3'-untranslated region of TP53 contributes to development of age-associated cataracts by modifying microRNA-125b-induced apoptosis of lens epithelial cells.
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