|Western blot, immunohistochemistry, Caspase assay, TUNEL assay, siRNA injection
|Retina-RPE separation resulted in a Fas-dependent activation of autophagy, with increased Atg5 levels and intraphotoreceptor conversion of LC3-I to LC3-II. Inhibition of autophagy by 3-MA or siAtg5 accelerated the time course of caspase 8 activation and photoreceptor TUNEL staining.
|Autophagy activation in the injured photoreceptor inhibits fas-mediated apoptosis.
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