|human trabecular meshwork (HTM) cells; human aortic smooth muscle cells (HASMC); human choroidal endothelial cells (HCEC); human RPE cell line ARPE-19
|transfection, phalloidin staining, qRT-PCR, luciferase reporter assay
|Targeted deletion of miR-143/145 in mice results in significantly reduced IOP, consistent with an ~2-fold increase in outflow facilities.We found that miR-143/145 regulates actin dynamics and the contractility of TM cells, consistent with its regulation of actin-related protein complex (ARPC) subunit 2, 3, and 5, as well as myosin light chain kinase (MLCK) in these cells.
|Regulation of intraocular pressure by microRNA cluster miR-143/145.
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