|retinal ganglion cell line RGC-5
|siRNA transfection, flow cytometry, qPCR, Western blot
|ATF3 specific siRNA effectively down-regulated ATF3 expression and significantly inhibited cell apoptosis in RGC-5 cells. Quantitative real-time PCR and Western blot confirmed that ATF3 knockdown remarkably decreased Jun-B and increased c-Jun at both mRNA and protein levels in RGC-5 cells.
|Effect of ATF3-deletion on apoptosis of cultured retinal ganglion cells.
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