|choroidal neovascularization (CNV)
|rhesus choroid-retinal endothelial cell line RF/6A
|RT-PCR, qPCR, Western blot, siRNA transfection, flow cytometry, MTT assay, transwell assay, tube formation assay
|Knockdown of DLL4 expression significantly promoted the proliferation of hypoxic RF/6A cells and led to their arrest in the S phase of the cell cycle. Migration and tube formation of hypoxic RF/6A cells were significantly induced by the DLL4 siRNA, with the number of migrated cells increased by 1.6-fold and total tube length increased by 82.3%, compared with the scrambled siRNA.
|Effect of DLL4 siRNA on proliferation, migration and tube formation of choroid-retinal endothelial cells under hypoxic conditions.
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