|choroidal neovascularization (CNV)
|human umbilical vein endothelial cells (HUVECs)
|Taqman assay, transfection, Boyden chamber migration assay, Scratch wound migration assay, BrdU incorporation assay, qRT-PCR, Western blot, luciferase reporter assay, isolectin-B4 staining
|Our results identify miR-21 as a new angiogenesis inhibitor and suggest that inhibition of cell migration and tubulogenesis is mediated through repression of RhoB. The therapeutic potential of miR-21 as an angiogenesis inhibitor was demonstrated in vivo in a mouse model of choroidal neovascularization.
|MicroRNA-21 exhibits antiangiogenic function by targeting RhoB expression in endothelial cells.
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