|Pseudomonas aeruginosa keratitis
|corneas; mouse Langerhans cell line XS52
|qRT-PCR, siRNA transfection and injection, ELISA, immunostaining
|LCs stimulated with CpG DNA and treated with TLR9 siRNA exhibited reduced TLR9, IL-1beta, and MIP-2 levels compared with controls. Finally, B6 mice treated with TLR9 siRNA showed decreases in corneal opacity, polymorphonuclear leukocyte number, IL-12 and IFN-gamma mRNA, IL-1beta, and MIP-2 protein compared with those treated with control siRNA.
|Silencing Toll-like receptor-9 in Pseudomonas aeruginosa keratitis.
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